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23710A

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Image #   23710A
Name:   Methylotrophic yeast (Komagataella pastoris)
Copyright:   Copyright 2004 Dennis Kunkel Microscopy, Inc.
Description:   Caption: Methylotrophic yeast (Komagataella pastoris, fromerly known as Pichia pastoris). This methylotrophic yeast was isolated from nature and can consume methanol as a sole carbon source when glycerol or glucose is not found as a food source. It is a recombinant organism that is used to produce large quantities of specialized proteins. To metabolize methanol Komagataella pastoris has an alcohol oxidase (AOX) gene. The K. pastoris expression system uses the methanol-induced alcohol oxidase promoter (AOX1) which controls the gene that codes for the expression of alcohol oxidase (the enzyme which catalyzes the fist step in the metabolism of methanol). Using genetic techniques genes for pharmaceutical proteins can be inserted next to the AOX-gene in Komagataella allowing the yeast to produce the protein. A number of proteins have been produced using this system, including tetanus toxin fragment, Bordatella pertussus petactin, human serum albumin and lysozyme.
Magnification*: x1,600
Type: SEM
    *(Magnifications are based on a 35mm slide image of 24mm in the narrow dimension.)
Keywords:   23710A 06.01.04 alcohol oxidase benchtop fermentation bud bud scar bud scars budding eukaryote eukaryotic expression fungi fungi and slime molds fungus gene expression genetic recombination methanol fermentation methylotrophic yeast Pichia pastoris Komagataella pastoris protein expression recombinant organism recombinant yeast yeast yeast bud yeast budding SEM enzyme enzyme production enzymes human growth hormone human growth hormones protein production proteins recombinant DNA recombinant DNA biotechnology recombinant DNA technology

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